Selected publications
1485676
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modern-language-association
50
date
1
1
482
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Lubbe, Lizelle, et al. “Cryo‐EM Reveals Mechanisms of Angiotensin I‐converting Enzyme Allostery and Dimerization.” The EMBO Journal, vol. 41, no. 16, Aug. 2022, p. e110550, https://doi.org/10.15252/embj.2021110550. Cite
Makumire, Stanley, et al. “The Structures of the C146A Variant of the Amidase from Pyrococcus Horikoshii Bound to Glutaramide and Acetamide Suggest the Basis of Amide Recognition.” Journal of Structural Biology, vol. 214, no. 2, June 2022, p. 107859, https://doi.org/10.1016/j.jsb.2022.107859. Cite
Martin, Darren P., et al. “Selection Analysis Identifies Clusters of Unusual Mutational Changes in Omicron Lineage BA.1 That Likely Impact Spike Function.” Molecular Biology and Evolution, vol. 39, no. 4, Apr. 2022, p. msac061, https://doi.org/10.1093/molbev/msac061. Cite
Van Wyk, Jennifer C., et al. “Engineering Enhanced Thermostability into the Geobacillus Pallidus Nitrile Hydratase.” Current Research in Structural Biology, vol. 4, Jan. 2022, pp. 256–70, https://doi.org/10.1016/j.crstbi.2022.07.002. Cite
Masamba, Priscilla, et al. “Crystallization and Preliminary Structural Determination of the Universal Stress G4LZI3 Protein from Schistosoma Mansoni.” Informatics in Medicine Unlocked, vol. 32, Jan. 2022, p. 101057, https://doi.org/10.1016/j.imu.2022.101057. Cite
Tegally, Houriiyah, et al. “Detection of a SARS-CoV-2 Variant of Concern in South Africa.” Nature, vol. 592, no. 7854, Apr. 2021, pp. 438–43, https://doi.org/10.1038/s41586-021-03402-9. Cite
Egan, TJ, et al. “Fate of Haem Iron in the Malaria Parasite Plasmodium Falciparum.” Biochemical Journal, vol. 365, no. Pt 2, July 2002, pp. 343–47, https://doi.org/10.1042/BJ20020793. Cite