Preliminary Programme

Note: Programme subject to change!

Outline

2020-03-31
TUESDAY: Introductions; Basic concepts of diffraction crystallization and data collection
With all speakers and organisers. Opening reception and poster session. JUMP TO DETAILS 
2020-04-01
WEDNESDAY: Data collection, processing theory; Radiation damage; MX capabilities at Diamond Light Source; Molecular graphics

With Gwyndaf Evans, Elspeth Garman, Bernhard Lohkamp, Andrew Leslie, et al. JUMP TO DETAILS 

2020-04-02
THURSDAY: Data processing

With Andrew Leslie, James Parkhurst, Kay Diederichs, et al. JUMP TO DETAILS 

2020-04-03
FRIDAY: Data quality; Remote data collection

Everyone with crystals. With Kay Diederichs et al.. JUMP TO DETAILS 

2020-04-04
SATURDAY: Free day

Enjoy some sight-seeing.

2020-04-05
SUNDAY: Experimental Phasing, Model Building

With Pavol Skubak, Tim Grüne, et al. JUMP TO DETAILS 

2020-04-06
MONDAY: Molecular Replacement; Refinement; Model building at Lower Resolutions

With Garib Murshudov, Ronan Keegan, et al. JUMP TO DETAILS 

2020-04-07
TUESDAY: Ligands; Model Completion and Quality

With Garib Murshudiv, Robbie Joosten, Kay Diederichs, et al. JUMP TO DETAILS 

2020-04-08
WEDNESDAY: Beyond X-ray Crystal structures; Feedback

With all speakers and organisers. JUMP TO DETAILS 


Detailed Programme

Colour Code

Lecture session
Questions and answers and one-on-one problem-solving
Tutorials
Meals and Coffee breaks
Other

March 31

Introductions; Basic concepts of Diffraction Crystallization and Data collection

All lectures and breaks will be in Electromechanical Building (EM Building), room 1.08 (lower level), Upper Campus, University of Cape Town.

08:15 – 08:40 Check-in. Continued throughout the day Science Connect
08:40 – 08:50 Welcome by UCT leadership? Trevor? Management?
08:50 – 09:10 Opening notes and introduction Organizers
09:10 – 09:30 Speaker self-introductions All speakers
09:30 – 11:00 Overview of Projects and Problems All participants
11:00 – 12:00 Roadmap for crystal structure determination, CCP4 cloud E. Krissinel
12:00 – 13:30 Lunch
13:30 – 15:00 Basic Theory of Diffraction, Crystals, symmetry A. Leslie
15:00 – 16:30 Reciprocal space, Ewald sphere, Concepts and definitions in data collection TBD
16:30 – 16:45 Q/A and Discussion. All topics of the day All participants
16:45 – 17:00 Break
17:00 – 17:30 Students put up posters All students
18:00 – 21:00 Opening reception and poster session All participants

April 1

Data Collection and Processing theory; Radiation damage; MX capabilities at Diamond Light Source; Molecular Graphics

All lectures and breaks will be in Electromechanical Building (EM Building), room 1.08 (lower level), Upper Campus, University of Cape Town.

09:00 – 11:00 Synchrotron beamlines from MX. New generation detectors. TBD
11:00 – 11:15 Q/A and discussion All participants
11:15 – 11:30 Break
11:30 – 12:00 Some practical tips for data collection TBD
12:00 – 13:30 Lunch
13:30 – 14:15 MX capabilities at Diamond Light Source G. Evans
14:15 – 15:15 Radiation damage, Cryoprotection E. Garman
15:15 – 15:30 Q/A and discussion All participants
15:30 – 15:45 Break
15:45 – 16:45 Coot B. Lohkamp
16:45 – 18:15 General theory of data processing A. Leslie
18:15 – 19:00 Plan data collection for April 3 All with crystals and volunteer speakers
19:00 – 20:00 Dinner

April 2

Data processing

All lectures and breaks will be in Electromechanical Building (EM Building), room 1.08 (lower level), Upper Campus, University of Cape Town.

09:00 – 09:45 Data collection for experimental phasing TBD
09:40 – 10:45 Data quality K. Diederichs
10:45 – 11:00 Break
Rest of day Data collection All with crystals
12:00 – 13:30 Lunch
16:00 – 16:15 Break
19:00 – 20:00 Dinner
20:00 – Data collection All with crystals

April 3

Data Quality; Remote Data Collection

All lectures and breaks will be in Electromechanical Building (EM Building), room 1.08 (lower level), Upper Campus, University of Cape Town.

09:00 – 09:45 Data collection for experimental phasing TBD
09:40 – 10:45 Data quality K. Diederichs
10:45 – 11:00 Break
Rest of day Data collection All with crystals
12:00 – 13:30 Lunch
16:00 – 16:15 Break
19:00 – 20:00 Dinner
20:00 – Data collection All with crystals

April 4

Free Day


April 5

Experimental Phasing, Model Building

All lectures and breaks will be in Electromechanical Building (EM Building), room 1.08 (lower level), Upper Campus, University of Cape Town.

09:00 – 10:00 General theory of experimental phasing P. Skubak
10:00 – 10:50 SHELX tutorial T. Grune
10:50 – 11:00 Q/A and Discussion All participants
11:00 – 11:15 Break
11:15 – 12:15 Crank2 tutorial P. Skubak
12:15 – 13:45 Lunch
13:45 – 14:45 Model Building with ARP/wARP TBD
14:45 – 15:00 Q/A and Discussion All participants
15:00 – 16:00 Problem solving Student data
16:00 – 16:15 Break
16:15 – 19:00 Problem-solving Student data
19:00 – 20:00 Dinner
20:00 – 22:00 Problem-solving Student data

April 6

Molecular Replacement; Refinement; Model building at Lower Resolutions

All lectures and breaks will be in Electromechanical Building (EM Building), room 1.08 (lower level), Upper Campus, University of Cape Town.

09:00 – 10:00 Molecular Replacement R. Keegan
10:00 – 10:45 Molecular Replacement Tutorial R. Keegan
10:45 – 11:00 Q/A and Discussion All participants
11:00 – 11:15 Break
11:15 – 12:00 Model Building at Lower Resolutions: ISOLDE T. Croll
12:00 – 13:30 Lunch
13:30 – 14:30 Refinement Theory G. Murshudov
14:30 – 15:30 Refinement with CCP4 (REFMAC) and Tutorial G. Murshudov
15:30 – 15:450 Q/A and Discussion All participants
15:45 – 16:00 Break
16:00 – 19:00 Problem-solving Student data
19:00 – 20:00 Dinner
20:00 – 22:00 Problem-solving Student data

April 7

Ligands; Model Completion and Quality

All lectures and breaks will be in Electromechanical Building (EM Building), room 1.08 (lower level), Upper Campus, University of Cape Town.

09:00 – 09:40 Ligands and ligand dictionary G. Murshudov
09:40 – 10:20 Model completion R. Joosten
10:20 – 11:00 Validation, Deposition, Publication R. Joosten
11:00 – 11:15 Break
11:15 – 12:15 Model quality K. Diederichs
12:15 – 13:45 Lunch
13:45 – 14:00 Q/A and Discussion All participants
14:00 – 16:00 Problem-solving Student data
16:00 – 16:15 Break
16:15 – 19:00 Problem-solving Student data
19:00 – 20:00 Dinner
20:00 – 22:00 Problem-solving Student data

April 8

Beyond X-ray Crystal structures; Feedback

All lectures and breaks will be in Electromechanical Building (EM Building), room 1.08 (lower level), Upper Campus, University of Cape Town.

09:00 – 09:45 Analysis of macromolecular complexes (PISA) E. Krissinel
09:45 – 10:45 Introduction to CryoEM G. Murshudov
10:45 – 11:00 Break
11:00 – 12:00 Introduction to Electron Diffraction T. Gruene
12:00 – 12:15 Q/A and Discussions As needed
12:15 – 13:45 Lunch
13:45 – 14:00 Start backups All participants
14:00 – 15:00 Student feedback All participants
15:00 – 15:15 Concluding remarks from the organizers and Speakers Organizers and Speakers
15:45 – 16:00 Break
16:00 – 19:00 Unaddressed questions, last minute problem-solving As needed
19:00 – 20:00 Dinner

Fin